离子注入突变藻株在城市污水中的产油特性

摘要

考察了蛋白核小球藻出发株及其离子注入突变株在实际城市污水中的生长情况和产脂性能,以及对污染物的去除能力.结果表明,突变藻株在污水中油脂产率为32.5mg/(L·d),相比于出发株分别显著提高了23.81％(P＜0.05).通过对生成的脂肪酸甲酯进行气相色谱分析,结果显示离子注入诱变并未改变小球藻脂肪酸成分,但提升了单不饱和脂肪酸的含量,有利于改善所得生物柴油的品质培养结束后出水水质可达到一级A排放标准,相较于出发株,突变株对污水中污染物具有更好的降解效果.利用扫描电镜观察可以发现出发株和突变株藻细胞形态差异较小,元素含量分析发现突变株H/C值相比出发株有所下降,系统发育树构建说明突变株与出发株属于同种变异.%The growth and lipid productivity of original Chlorella pyrenoidosa and its ion implantation mutant strains were studied.The results showed that the lipid productivity of the mutant strain was 32.5mg/(L·d),23.81％ higher than that of the original strain.According to the fatty acid methyl ester analysis by gas chromatography,ion implantation did not change the composition of fatty acid,but enhanced the content of monounsaturated fatty acids,which was conducive to the enhancement of biodiesel quality.After cultivation,the effluent quality was also found to reach the emission standard of first level A,and the mutant showed better degradation ability than the original strain.Phylogenetic tree showed that the mutant and the original strains were belonged to interspecific variation.