ELISA检测兰州不同动物粪便中轮状病毒抗原及临床意义

摘要

为探讨动物轮状病毒检测的临床价值及本地轮状病毒在不同动物中的流行情况，本研究以秋冬季腹泻的动物为研究对象进行采样，应用轮状病毒单克隆抗体检测其抗原的方法，检测粪便中的轮状病毒。在进行牛、羊、犬粪便轮状病毒检测中发现犬的阳性率最高，其次是牛和羊。表明本地的轮状病毒感染主要集中在幼犬中。本试验为今后动物轮状病毒在临床应用中的检测及动物轮状病毒检测试剂盒的研制等作以参考。%The objective of this paper was to probe into clinical value of animal Rotavirus examination and the popular situation of local Rotavirus among different animals. Taking fall-winter diarrhea animal as the research subject to take sample, the method for using the Rotavirus monoclonal antibody was applied to detect its antigen and examine Rotavirus in animal excrement. In the detection of Rotavirus in excrement of cattle, sheep and dog, the result showed that dog＇s masculine gender rate was the highest, and the cattle＇s and sheep＇s arc the next. This finding indicated that local rotavirus mainly concentrates in dogs, which offered a reference for the examination of animal rotavirus in clinical practice and the development of animal rotavirus examination reagent box in the future.