Comparison of the serologic tests of indirect Immunofluorescence, rapid conglutination, indirect ELISA and competition ELISA for detection of antibodies against Anaplasma marginale in cattle sera from different enzootic areas

摘要

Serological tests like rapid conglutination test (RCT), indirect ELISA (iELISA) and indirect immunofluorescent assay (IFA) were comparatively evaluated to detect antibodies against Anaplasma marginale. Competitive ELISA was used as the gold test during the study. A total of 453 sera from vaccinated and unvaccinated cattle collected from enzootic stable and unstable areas were tested. iELISA, IFA and RCT presented 0.77 (substantial), 0.57 and 0.49 (moderate) kappa index; 90.6, 90.2 and 73.7% sensitivity; and 86.6, 62.8 and 79.3% specificity, respectively. Results showed that iELISA presented better specificity than IFA and RCT, and can be indicated for more detailed serological investigations, detection of persistently infected animals in cattle herds and for monitoring of vaccination programmes. IFA and RCT can be used in prevalence studies and to monitor cattle movement between different geographical regions..