母婴血型不合导致新生儿溶血病的实验室诊断技术分析

摘要

Objective To investigate the clinical value of the blood group Objective To investigate the clinical value of the blood group distribution of neonatal hemolytic disease (HDN) caused by maternal fetal blood group incompatibility, and to analyze its diagnostic value. Methods Selected in our hospital in April 2014 and 2015 years 4 months of submission of 420 patients with suspected HDN blood samples as the research object, the of ABO and Rh blood group identification and its direct anti human globulin, free lfuid antibody and elution antibody level detection. Results The blood group serological detection due to blood group incompatibility and cause hemolytic disease of the newborn of 242 patients, the positive rate of 57.6%, the ABO hemolytic disease of newborn with 240 cases (57.1%), and Rh-HDN have 2 cases (0.47%). In the 71 cases of O type blood, the direct immunoglobulin and irregular antibodies were all negative for detection. the 240 patients with ABO-HDN, the type A was 104 (43.3%) and B was 136 (56.7%). Conclusion The study of the blood type incompatibility and lead to neonatal hemolytic disease, ABO-HDN is common, and the mother of O blood type blood B blood newborn more see. The diagnosis and treatment of hemolytic disease of the newborn has important clinical signiifcance for the diagnosis and treatment of hemolytic disease of newborn.%目的：探讨临床母婴血型不合引发新生儿溶血病（HDN）的血型分布，分析其诊断技术对临床的价值。方法选取本院2014年4月～2015年4月送检的420例疑似HDN患儿血液样本作为研究对象，对其进行ABO和Rh血型鉴定，检测其直接抗人球蛋白、游离液抗体及放散液抗体的水平。结果采用血型的血清学检测检测出由于血型不合而引发新生儿溶血病的有242例，阳性率为57.6%，其中ABO-HDN的有240例（57.1%），而Rh-HDN的有2例（0.47%）。在71例O型血患儿中，采用直接抗球蛋白和不规则抗体检测时均为阴性。已证实为ABO-HDN的240例患儿中，A型的为104例（43.3%）， B型的为136例（56.7%）。结论本次研究的由于血型不合而导致新生儿溶血病中，ABO-HDN较为常见，而其中以母亲为O型血的B型血新生儿较多见。通过对新生儿溶血病的血型相符度进行检测，对新生儿溶血病的诊断及治疗具有重要的临床意义。