IRF-2在胰腺癌中的功能及其对胰腺癌细胞化疗敏感性的影响

摘要

Objective The purpose of the analysis and discussion of IRF-2 in pancreatic function and its chemosensitivity of pancreatic cancer cells. MethodsTo detect METT gemcitabine in pancreatic cancer cell lines MIAPaCa-2 and PANC-1 half measurement of these two cells to understand the sensitivity of chemotherapy for pancreatic cancer cells, select the cells more resistant to IRF-2 in pancreatic cancer cells chemosensitivity be further explored. Results MIAPaCa-2 and PANC-1 cells exist in IRF-2 gene expression, PANC-1 cells than MIAPaCa-2 high, gemcitabine MIAPaCa-2 cells median effective dose was significantly lower than PANC-1, P<0.05, drugs for 72h after interference IRF-2-expressing cells IC50 values below PANC-1 control cells, which showed that downregulation of IRF-2 gene, to enhance the PANC-1 cells to pancreatic cancer cell sensitivity to chemotherapy.Conclusion The study shows that, IRF-2 specific interference technology can effectively enhance chemosensitivity PANC-1 pancreatic cancer cells to gemcitabine.%目的：分析与探讨IRF-2在胰腺癌中的功能及其对胰腺癌细胞化疗敏感性的影响。方法采用METT检测吉西他滨对胰腺癌细胞株MIAPaCa-2与PANC-1的半数计量，对以上两种细胞对胰腺癌细胞化疗敏感程度加以了解，选择较为耐药的细胞对IRF-2对胰腺癌细胞化疗敏感性的影响进行进一步探讨。结果 MIAPaCa-2与PANC-1细胞中均存在IRF-2基因表达，PANC-1细胞中比MIAPaCa-2高，吉西他滨对MIAPaCa-2细胞半数有效剂量明显低于PANC-1，P＜0.05，药物作用72 h后，干扰IRF-2表达细胞IC50值低于PANC-1对照细胞，由此表明，IRF-2基因表达下调后，提升了PANC-1细胞对胰腺癌细胞化疗敏感性。结论研究表明，IRF-2干扰技术特异性能够有效提升PANC-1胰腺癌细胞对吉西他滨的化疗敏感性。