肝细胞肝癌中极性蛋白AF-6mRNA的表达及对侵袭的影响分析

摘要

目的：探讨肝细胞肝癌中极性蛋白AF-6mRNA的表达及其对侵袭的影响。方法采用实时定量PCR检测的方法，对28对癌细胞、ANLT及不同细胞系中的AF-6mRNA表达水平进行检测，分析AF-6mRNA对侵袭的影响。结果26例肝细胞肝癌中AF-6mRNA呈现低表达，发生率为92.9%；肝癌细胞株中AF-6mRNA表达低于正常杆细胞株（P＜0.05）；低侵袭转移能力细胞系中AF-6mRNA表达高于高侵袭转移能力细胞系（P＜0.05）。结论肝细胞肝癌中AF-6mRNA低表达可能与高侵袭能力有关。%Objective To investigate the expression of AF-6mRNA protein and its effect on the invasion of hepatocellular carcinoma.Methods Using real-time quantitative PCR detection method, the expression levels of AF-6mRNA in 28 pairs of cancer cells, ANLT and cell lines were detected, and the inlfuence of AF-6mRNA on the invasion was analyzed.Results 26 cases of AF-6mRNA showed low expression in hepatocellular carcinoma, the incidence rate was 92.9%, the rod was signiifcantly lower than the normal cell line AF-6mRNA expression in hepatocellular carcinoma cell line (P<0.05), was obviously higher than that of invasion and metastasis of AF-6mRNA cells expressing ability in cell lines with low metastasis (P<0.05). ConclusionLow expression of AF-6mRNA in hepatocellular carcinoma may be related to high invasive ability.