从沂蒙地区灌木枯枝中筛选到一株产纤维素酶菌株,初步鉴定为绿色木霉(T.viride Persex Fx NS90).该研究考察了不同浓度阻遏剂甘油、葡萄糖、纤维二糖对菌株生长和产酶的影响.结果显示:菌株生长的生物量随发酵培养基中甘油、葡萄糖、纤维二糖的浓度增加而提高,当培养基中分别含0.6%的甘油或0.4%葡萄糖时,菌株的相应发酵产酶活力较高,葡萄糖的浓度达2%时,菌株发酵产酶能力受到明显抑制,纤维二糖浓度对菌株发酵产酶没有明显的影响.实验采用紫外和微波对筛选菌株进行诱变处理,在含一定浓度阻遏剂的分离培养基上进行修复筛选,对选育出突变菌株的显微形态、抗阻遏性能和发酵产酶能力进行考察.结果表明:经紫外照射90s,微波辐射60s,结合2%葡萄糖平板修复选育,筛选到的突变株在生长过程中,菌落由黄色逐渐变为绿色,呈现黄色孢子,相对于出发菌株其抗阻遏性能明显提高,摇瓶发酵产CMCA和FPA酶活分别提高了41.0%和44.95%.%A cellulase -producing strain, which was identified as Trichoderma viride (T.viride Persex Fx NS90), was isolated from sapless shrubs in Yimeng area.Effects of different concentrations of glycerol, glucose, cellobiose on growth of T.viride and enzyme production were studied.The results indicated that biomass of strain growing positively correlated with concentrations of glycerol, glucose, cellobiose in the medium.The yield of cellulase was the highest when the media added 0.6％ of glycerol or 0.4％ glucose, and addition of 2％ glucose in medium significantly inhibited cellulase production, and addition of cellobiose had no effect on yield of cellulase.The start strain was mutated with UV and microwave and the strain was repaired and screened in medium containing inhibitors.Colony morphology, anti - inhibit ability and cellulase - producing capacity of mutant strain were investigated.The results showed that, after mutated with combination of 90s UV radiation and 60s microwave, repair and screening with plate media had 2％ glucose, a mutant strain was obtained.The color of mutant colony changed gradually from yellow to green, yellow spores occurred during innoculation.The ability of CMCA and FPA production of the mutant strain increased 41.0％ and 44.95％, respectively when compared with starting strain.
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