Quantitative determination of quercetin, morin hydrate, kaempferol, isorhamnetin, luteolin, apigenin in red wine with high performance liquid chromatography (HPLC) with ternary gradient was established. The flavonoids were separated by HPLC with Luna C18(4.6mm× 150mm, 3μm)and a Phenomenex C18 precolumn(4mm×3.0mm) at 50℃. Mobile phase A, B and C were acetonitrile, ethanol and 0.2％ H3PO4, respectively. Gradient elution rate was 0.25ml/min and detection wavelength was 265nm. The results showed that the calibration curves were linear at ranges of 0.7mg/L～32.0mg/L. The average recovery in the between of 98.2 ％ ～ 101.1％ and RSD 1.88 ％ ～2.85 ％.The method is simp le and accurate with good reproducibility and can be used in quantity determination of flavonoid aglycone of natural product.%建立了三元梯度的高效液相色谱法(HPLC)测定红酒中的桑色素、槲皮素,山奈酚、异鼠李素、木犀草素、芹菜素等苷元的含量的方法.采用Luna C18(4.6mm×150mm,3μm)色谱柱,预柱(4mm×3.0mm),柱温为50℃,流动相:A相乙腈,B相乙醇,C相为0.2%磷酸水溶液.流速为0.25mL/min,梯度淋洗,检测波长265nm.结果显示:各黄酮苷元在0.7mg/L～32.0mg/L呈线性关系,样品的加标平均回收率为98.2%～101.1%,RSD为1.88%～2.85%,该方法简便、准确,可用于天然产物中黄酮苷元的含量的测定.
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