地衣芽孢杆菌BL-5芽孢生成条件优化

摘要

Bacillus licheniformis,as micro-ecological agent,has broad application prospects in food and feed industry.The effect of temperature on B.licheniformis BL-5 lethality was studied.The results showed that lethal temperature of spore-free bacteria was 75 ℃.According to the sporulation rate and spores number,the optimal medium formula for the sporulation rate ofB.licheniformis BL-5 was as follows:sucrose 29.85 g/L,ammonium sulphate 10.00 g/L,yeast extract 3.00 g/L,dipotassium phosphate 3.42 g/L,magnesium sulfate 1.5 g/L,pH 8.0.The optimal culture conditions were as follows:inoculum 5％,fermentation time 48 h,fermentation temperature 30 ℃,and rotation speed 150 r/min.%地衣芽孢杆菌(Bacillus licheniformis)作为微生态制剂在食品或饲料工业中具有广泛的应用前景.通过温度对地衣芽孢杆菌BL-5致死率的影响研究得出无芽孢菌体的致死温度为75℃.以芽孢率和芽孢数为评价指标,得到地衣芽孢杆菌BL-5芽孢生成的最佳培养基配方为:蔗糖29.85 g/L、硫酸铵10.00 g/L、酵母膏3.00 g/L、磷酸氢二钾3.42 g/L、硫酸镁1.5 g/L、pH 8.0.最佳培养条件为:接种量5％、发酵时间48 h,发酵温度30℃、转速150 r/min.