To establish canine acute pancreatitis model, the compounds trypsin and sodium cholate were injected into canals of Wirsung total for 4 dogs. When the acute pancreatitis model were challenged, observe its pathological changes in breast tissue morphology, HCT, WBC, AMY, positive result of blood culture on 1st day, 2nd day, 4 th day and 7th day, and 7 days later. The results showed that ①Severe hyperaemia and haemorrhage of pancreas could be seen after injecting sodium cholate.②In comparison with those of HCT, WBC, AMY, the animals were significantly higher than pre-challenge level. The results demonstrated that the case model of acute pancreatitis could be built by injecting sodium cholate and compounds trypsin.%为建立犬急性胰腺炎模型,本试验选用4只本地健康杂交犬作为研究对象,采用胰管逆行注射法即在主胰管注入牛磺胆酸钠与胰蛋白酶复合液构建急性胰腺炎模型.建模后,观察其组织形态学变化,并于术后1、2、4、7d动态地检测其红细胞压积、白细胞总数、淀粉酶含量的变化.结果显示:①在向主胰管内注入牛磺胆酸钠与胰蛋白酶复合液5～10 min可看到胰腺充血、出血严重.②试验动物的红细胞压积、白细胞总数以及淀粉酶含量在术后均有显著变化.结果证明采用主胰管内注入牛磺胆酸钠与胰蛋白酶复合液可以构建稳定的急性胰腺炎病理模型.
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