链球菌GaPc基因检测及其序列分析

摘要

根据已发表的猪链球菌2型3-磷酸甘油醛脱氢酶(Gapc)基因保守区域设计并合成l对特异性引物,对10株不同来源链球菌Gapc基因进行PCR扩增,并选择PCR产物进行克隆并测序分析,结果在10株不同来源链球菌菌株中有8株可扩增出与预期大小(1011 bp)相一致的DNA片段;选取4株细菌PCR产物进行克隆测序,发现其片段大小为1009或1012bp,与GenBank参考菌株Ga pc基因序列的核苷酸同源性为85.3%～98.3%,氨基酸同源性为87.2%～99.4%;生物学软件分析结果显示Ga pc 基因在链球菌属细菌中相对保守,且具有较多的潜在抗原表位.本研究为链球菌检测技术和核酸疫苗的研究奠定了基础.%A pair of primer was synthesized based on the published Streptococcus suis type 2 glyceraldehyde-3-phosphate dehydrogenase gene (Gapc) and PCR was applied to detect 10 Streptococcus strains. The amplified products were cloned and sequenced. The results showed that the Gapc gene of 8 Streptococcus strains was detected in all tested strains. The Gapc gene of the above 4 strains were sequenced and analysised by BLAST and DNAStar with pubished Streptococcus Gapc in GenBank. The nucleotide homology of them was 85.3％ to 98. 3％ and the amino acid homology was 87.2％ to 99.4％. It had many domains of high antigen index and hydrophilicity and surface probability plot and Gapc gene in Streptococcus was conservative. The results indicated that the Gapc may be applied into clinical prevention for Streptococci infections.