为研究穿心莲水提物与临床常用10种抗菌药联用对鸡致病性大肠杆菌的体外抑菌效果,本试验采用传统的水提法制备穿心莲中药液并浓缩至浓度为1 g/mL,用琼脂平板稀释法测定穿心莲水提物分别与阿莫西林、头孢曲松等10种常用抗菌药物联用对临床分离的10株鸡致病性大肠杆菌的体外抑菌作用.结果表明,穿心莲和头孢曲松、穿心莲和氟苯尼考联用100％呈现协同作用;穿心莲和头孢噻呋联用90％呈现协同作用,10％呈现无关作用;穿心莲和大观霉素联用80％呈现协同作用,20％呈现无关作用;穿心莲和林可霉素联用50％呈现协同作用,40％为无关或颉颃作用,10％为无关作用;穿心莲与阿莫西林、安普霉素、阿米卡星、多西环素、恩诺沙星联用以无关或颉颃作用为主.以上结果表明,在体外,穿心莲与头孢曲松、头孢噻呋、大观霉素、氟苯尼考联用对鸡致病性大肠杆菌呈现协同作用,与阿莫西林、安普霉素、阿米卡星、林可霉素、多西环素、恩诺沙星联用呈现无关或颉颃作用.%To study the antibacterial effect in vitro of Andrographis aqueous extract associated with clinical qommonly used 10 kinds of antimicrobial agents to the chicken pathogenic Escherichia colt. Andrographis prepared by the traditional water extraction liquid and concentrated to a concentration of 1 g/mL,determined the invitro inhibitory effect of Andrographis aqueous extract respectively, associated with amoxicilhn, ceftriaxone, and other 10 kinds of commonly used clinical antimicrobial drugs, using the agar dilution method, to the clinical 10 chicken pathogenic Escherichia coli. The results show that Andrographis paniculata and ceftriaxone, Andrographis paniculata and florfenicol associated with 100% showing a synergistic effect; Andrographis paniculata and ceftiofur associated with 90% showing a synergistic effect, 10% showing unrelated; Andrographis paniculata and spectinomycin combined with 80% showing synergies, 20% showing unrelated; Andrographis paniculata and lincomycin associated with 50% showing a synergistic effect, 40% for unrelated or antagonistic effects, of 10% for unrelated; Andrographis paniculata with amoxicillin, apramycin, amikacin, doxycycline, enrofloxacin linked to unrelated or antagonistic effects mainly. The above results revealed that, in vitro, Andrographis paniculata associated with ceftriaxone, ceftiofur, spectinomycin, florfenicol showing synergy to chicken pathogenic Escherichia coli, with amoxicillin, apramycin, amikacin, lincomycin, doxycycline, enrofloxacin with unrelated or antagonistic effects.
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