理囊散对不孕奶牛血清中微量元素和白介素影响的研究

摘要

The assay was aimed to investigate the law of trace elements (Cu, Fe, Mn, Zn) and interleukin (IL-1, IL-6) contents in scrum using Cyst-regulating powder to treat infertility caused by ovarian disease of dairy cattle. Flame atomic absorption spectrometry and enzyme-linked immunization absorb assay were used to detect levels of the trace elements and interleukin contents in serum from the experimental and normal group before and after treatment. The results were showed as following. ①After treatment, the effective rate of follicular cyst was 83.33%, the effective rate of delayed ovulation was 90. 91%.②In follicular cyst body, Mn, Zn contents were significantly lower than the normal group in pre-treatment (P< 0. 05),Fe content was significantly higher than the normal group(P<0. 05), after treatment, their content levels were closed to the normal group, and the difference was not significant(PX). 05), the Cu, IL-1, IL-6 contents were closed to the normal group before and after treatment. ③In delayed ovulation, Fe content was signifcantly higher than the normal group(P<0. 05), Mn content was extremly significantly lower than the normal group in pre-treatment(P<0. 01), Zn content was signifcantly higher than the normal group (P<0. 05), after treatment, the contents was not gradually significant(P>0. 05),but Cu, IL-1, IL-6 content levels were not significantly compared with the normal group before and after treatment(PX). 05). In conclusion, the effect of trace elements and interleukin in serum can't be ignored by using Cyst-regulating powder to treat infertility caused by ovarian disease of dairy cattle.%本试验旨在探讨经理囊散治疗不孕症奶牛前后,血清中微量元素(Cu、Fe、Mn、Zn)与白介素(IL-1、IL-6)的含量变化规律.利用火焰法原子吸收光谱法和酶联免疫吸附法分别检测试验组和对照组治疗前、后血清中微量元素和白介素的含量.试验结果表明,①理囊散治疗卵泡囊肿有效率为83.33％,排卵延迟有效率为90.91％;②在卵泡囊肿型奶牛中,Mn、Zn含量治疗前均显著低于正常组(P＜0.05),Fe治疗前显著高于正常组(P＜0.05),经治疗后逐渐达到正常值水平,Cu、IL1、IL-6含量治疗前后差异不显著；③在排卵延迟型奶牛中,Fe治疗前显著高于正常组(P＜0.05),Mn含量治疗前极显著低于正常组(P＜0.01),Zn含量治疗前显著低于正常组(P＜0.05),经治疗后逐渐达到正常值水平,Cu、IL-1、IL-6含量治疗前后差异不显著.因此,理囊散对奶牛不孕症血清中部分微量元素和白介素含量有微调作用,在奶牛不孕症治疗效果上有不可忽视的作用.