兔防御素基因NP-1的克隆及其在大肠杆菌中的表达

摘要

The mature peptide sequence of rabbit defensin NP-l gene was modified with favour codes for E. colt and synthesized chemically. The target gene fragment modified was cloned into vector T-easy,and then inserted into fusion expression plasmid pMAL-p2X through digesting of two restriction enzymes. The positive recombinant expression vector pMAL-NP-1 was transformed into E. colt TB1, which could effectively produce the fusion protein MBP and NP-l induced with IPTG.%选用大肠杆菌偏嗜性的密码子对兔防御素基因NP-1进行改造,人工合成编码NP-1成熟肽的基因片段,并将其克隆到T-easy载体上,再经双酶切后重组于原核表达载体pMAL-p2X.经IPTG诱导后,在大肠杆菌TB1中融合表达出兔防御素NP-1成熟肽.