一株鸡传染性法氏囊病病毒的分离鉴定

摘要

One isolate of infectious bursal disease virus (IBDV) ,was isolated from the vaccination failure chicken. Through serologic AGP experiment,results showed obvious white precipitation linei the high variable (hv) region of VPZ genes were amplified by RT-PCR (reverse transcriptase polymerase chain reaction) and were compared for their sequences. It was indicated that the homology of TJ-hg strain and very virulent IBDV strain all reached above 90% and 98. 8% to 99. 3% at nucleotide level, respectively. And the change of amino acid was complement in high variable (hv) region of VP2 genes. The mutations a-mino acid Q219P located in the first hydrophilic region of TJ-hg strain. The research demonstrated that TJ-hg strain belonged to vvIBDV,but it was different from standard strain.%从天津地区免疫失败的鸡群中分离一株IBDV(命名TJ-hg),应用血清学AGP试验结果可见明显的白色沉淀线；应用逆转录酶—聚合酶链式反应( RT- PCR)扩增的IBDV VP2基因高变区(vVP2)及其序列分析的结果表明,TJ-hg株与超强毒株的核苷酸同源性均在90％以上,氨基酸同源性达98.8％～99.3％,且高变区中特征性氨基酸的变化与超强毒株完全一致,TJ-hg株在第一亲水区氨基酸Q219P发生突变.研究结果表明,TJ-hg株属于超强毒株,但又与标准毒株存在差异.