杜洛克猪 HMGA1基因多态位点与生长、饲料利用性状的关联分析

摘要

试验对 HMGA1基因的两个 SNPs 位点进行分析,研究了 HMGA1基因核苷酸多态性与生长、饲料利用性状的关联性。利用大白猪和民猪重测序结果比较发现了 HMGA1基因的两个 SNPs 位点(g.-543 T > C 和g.1356 C>T),利用 Sequenom 质谱测序平台对杜洛克猪 g.-543 T>C 和 g.1356 C>T 位点进行基因分型,并分析该位点多态性与生长、饲料利用性状的关联性。结果发现,杜洛克猪群体 g.-543 T>C 位点,CT 与 CC 基因型相比,90日龄体重升高了2.15 kg (P <0.05),30 kg 体重日龄降低了3.21 d (P <0.05),断奶重升高,剩余采食量降低,100 kg 体重日龄减少,30~100 kg 平均日增重、40~90 kg 平均日采食量、40~90 kg 平均日增重均降低。杜洛克猪群体 g.1356 C>T 位点,CC 与 TT 基因型相比,90日龄体重升高了0.37 kg (P <0.05)；平均日采食量降低0.13 kg/d (P <0.05),100 kg 背膘厚降低0.43 mm (P <0.05),剩余采食量降低了70.42 g (P <0.05),30 kg 体重日龄降低了0.56 d (P <0.05)；40~90 kg 平均日采食量降低了0.17 kg (P <0.05),断奶重升高,100 kg 体重日龄增大,30~100、40~90 kg 平均日增重均降低；CT 与 TT 基因型结果与上结果类似。结果初步表明 HMGA1基因两个 SNPs 位点的突变有利于杜洛克猪的生长、饲料利用效率的提高。%The objective of this study was to analyze two SNPs of HMGA1 gene,and investigate its association with feed efficiency related traits in Duroc populations.HMGA1 gene SNPs (g.-543 T>C and g.1356 C>T)in Large White pig and Min pig were detected by re-sequen-cing technology,SNP genotyping of g.-543 T>C and g.1356 C>T sites in Duroc populations were performed using Sequenom mass spectrometry,and the association of HMGA1 gene SNPs with growth and feed efficiency related traits in Duroc populations were analyzed.The results in- dicated that the individuals of CT genotype in g.- 543 T > C increased 2.15 kg on 90 d body weight (P <0.05 ),decreased 3.21 d at 30 kg weight age (P <0.05 ),weaning weight was in-creased and 100 kg age were decreased,residual feed intake,30 to 100 kg average daily gain (ADG),40 to 90 kg average daily feed intake (ADFI)and 40 to 90 kg ADG were decreased,com-paring with CC individuals.Moreover the individuals of CC genotype in g.1356 C>T had signifi-cantly increased 90 d body weight (0.37 kg,P <0.05),ADFI (less 0.13 kg,P <0.05),back fat (BF,less 0.43 mm,P <0.05),RFI (less 70.40 g,P <0.05),30 kg age (less 0.56 d,P <0.05) and 40 to 90 kg ADFI (less 0.17 kg,P <0.05)were significantly decreased;Weaning weight and 100 kg age were increased;30 to 100 kg ADG,40 to 90 kg ADG were decreased than TT genotype individuals.And the CT genotype individual in g.1356 C>T had the similar results with CC gen-otype individuals that compared with TT genotype individuals.All in all,the two SNPs of HM-GA1 gene would be helpful in feed efficiency related traits in Duroc population.