复方党参口服液对免疫应激蛋雏鸡免疫分子的影响

摘要

本试验旨在研究以党参为君药制成的复方党参口服液对免疫应激状态下的蛋雏鸡血清 IgG、白细胞介素‐2和血清促肾上腺皮质激素（ACT H ）水平的影响。本试验将150羽体重相近健康的1日龄蛋雏鸡随机分成5组，每组30羽，即高剂量组、中剂量组、低剂量组、模型对照组和基础组，从3日龄开始，高、中、低剂量组分别按8、4、2 mg／kg体重饮水添加口服液，连续添加10 d。7日龄时高、中、低及模型对照组肌肉免疫注射新城疫Ⅰ系疫苗制造免疫抑制模型，基础组不做任何处理。分别于8、16、24、32、40日龄采血测定复方党参口服液对免疫分子的影响。结果显示，在16、24、32日龄时，高、中、低剂量组血清Ig G含量均极显著高于模型对照组（ P＜0.01），且3个剂量组中免疫抑制雏鸡血清IgG含量由高到低依次为：高剂量＞中剂量＞低剂量；在8、16日龄时，高、中剂量组血清IL‐2含量显著或极显著高于模型对照组（ P＜0.05；P＜0.01），24、40日龄时，3个剂量组均极显著高于模型对照组（P＜0.01），32日龄时，高剂量组极显著高于模型对照组（P＜0.01），中剂量组显著高于模型对照组（P＜0.01）；在8、16、24和40日龄时，高、中剂量组血清AC T H含量显著或极显著低于模型对照组（ P＜0.05；P＜0.01），32日龄时，高剂量组显著低于模型对照组（P＜0.05），试验后期3个剂量组和模型对照组的ACTH水平逐渐接近于基础组，说明免疫应激对ACT H的升高呈现一过性。综上所述，复方党参口服液能提高免疫应激蛋雏鸡血清中IgG和IL‐2水平，降低ACT H的水平，但是不能降低到基础组的正常水平，综合来看，高剂量组复方党参口服液效果最优。%The test was aimed at determining the effects of compound Codonopsis oral liquids with Codonopsis pilosula as monarch drug on levels of IgG ,IL‐2 and ACTH of serum with immuno‐logical stress in layer chickens .One hundred and fifty healthy chickens at one day old with the same body weight were divided into five groups of 30 chickens per group :High dose group ,medi‐um dose group ,low dose group ,model control group and base control group ,respectively .From the 3 days old ,the high ,medium and low dose groups were respectively administered 8 ,4 and 2 mg/(kg · BW) compound Codonopsis oral liquids for 10 days;The chickens of 3 different dose groups and model control group were intramuscularly injected Newcastle disease (ND) vaccinesⅠ to make immunosuppressive models on 7th day .The samples were collected and IgG ,IL‐2 and ACTH contents in serum were determined on the 8th ,16th ,24th ,32th and 40th day .The results showed that on the 16th ,24th and 32th day ,the IgG content of 3 different dose groups were ex‐tremely significantly higher than that of model control group (P<0 .01) ,and high dose group>middle dose group>low dose group .On the 8th and 16th day ,the IL‐2 level of high and middle dose groups were extremely significantly or significantly higher than that of model control group (P<0 .05 ;P<0 .01) ,and on the 24th and 40th day ,3 different dose groups were extremely signif‐icantly higher than that of model control group (P<0 .01) ,and that of high dose group was ex‐tremely significantly higher (P< 0 .01)while middle dose group was significantly higher (P<0.05)than the model control group on the 32th day ;On the 8th ,16th ,24th and 40th day ,the ACT H content of high and middle dose groups were extremely significantly or significantly lower than that of model control group (P<0 .05 ;P<0 .01) ,and that of high dose group was signifi‐cantly lower than model control group on the 32th day (P<0 .05) .At the late experiment period , the ACT H content of 3 different dose groups were gradually became similar to base control group ,in‐dicating that the effect of immunosuppressant on ACTH was transient .In conclusion ,the compound Codonopsis oral liquids could increase the level of levels of IgG ,IL‐2 and reduce the level of ACTH of se‐rum ,but it could not decrease to the level of base control group ,and high dose had better effects .