绒山羊次级毛囊基因表达滞后性的转录组分析

摘要

毛囊是具有高度的自我更新能力、独特的可再生器官，其周期性变化是一个动态过程，为了研究毛囊差异基因表达变化情况，文章以内蒙古绒山羊生长期毛囊为研究对象，利用 Illumina 高通量测序技术方法，检测6~10月皮肤毛囊差异基因表达变化情况，寻找对绒毛生长及质量具有调控作用的特异表达基因。试验发现，多数差异基因分别在初级毛囊(PF)的7、8月份和次级毛囊的(SF)8、9月份表达；在绒毛生长期的相邻月份中，发现FAM83C、LOC102185501、FYCO1、CTTNBP2NL、C6orf132、KRTAP29-1和CRYBG37个共同差异表达基因呈现相同的表达趋势，且在次级毛囊上的表达变化均比初级毛囊滞后1个月。综上所述，次级毛囊在基因表达调控中存在一定的滞后性，这将为绒毛周期生长发育和分子育种提供重要的理论基础。%Hair follicle is capable of self-renew and its growth cycle is a dynamic process.In order to investigate the vary of differential expression genes in the Nei Mongol cashmere goat hair folli-cles during anagen,the mRNA expression levels of different genes in skin hair follicles were de-tected from June to October using Illumina technology.Evaluation of transcriptomes was under-taken to understand the time-course gene expression involved in both follicle types.The samples were collected from June to October.Most of differentially expressed genes appeared between July to August in primary hair follicle (PF)and between August to September in secondary hair folli-cle (SF).Seven differentially expressed genes,FAM83C,LOC102185501,FYCO1,CTTNBP2NL, C6orf132,KRTAP29-1 and CRYBG3 were identified.Their expression demonstrated similar trends at different months of cashmere growth cycle and the SF postpone for about one month. These findings indicated the hysteresis in SF gene regulation comparing with PF,and could pro-vide the useful theoretical basis for the molecular mechanism of cashmere regeneration.