阿魏根粗多糖对鸡脾脏和外周血淋巴细胞体外增殖作用的影响

摘要

To investigate the effects of Ferula sinkiangensis polysaccharides (FSP) on cell proliferation of chicken splenic lymphocytes and peripheral lymphocyte in vitro.By the method of water extract-alcohol precipitation,total Ferula sinkiangensis polysaccharide (FSPt) and 30 ％,50 ％,70％ gradient alcohol precipitation of polysaccharides (FSP30,FSP50 and FSP70) were extracted,and detected by phenol-sulfuric acid method and infrared spectroscopy method.The safe concentration of all classifications of polysaccharide were detected and the effect of FSP on lymphocyte proliferation of chicken spleen and peripheral blood were measured by MTT method.The results showed that the absorption peak of FSP were conformed to belong by polysaccharide compounds.Peripheral blood T lymphocyte proliferation test indicated that FSPt,FSP30 and FSP70 alone or cooperated with PHA could stimulate T lymphocyte proliferation under a certain concentration,and FSPt showed a better proliferation effect.Spleen B lymphocyte proliferation test showed that FSPt,FSP30 and FSP70 alone or cooperated with LPS could stimulate B lymphocyte proliferation under a certain concentration,FSPt and FSP70 showed a better proliferation effect.In conclusion,FSP showed the strong effect on lymphocyte proliferation of chicken spleen and peripheral blood in vitro,and that would be the materials for further research.%为研究新疆阿魏多糖(FSP)对鸡外周血T淋巴细胞和脾脏B淋巴细胞增殖作用的影响.本研究用水提醇沉法得到阿魏总多糖(FSPt)和3种分级多糖(FSP30、FSP50、FSP70),并通过苯酚—硫酸法和红外光谱对FSP进行检测.通过MTT法测定各级FSP的安全浓度及其对鸡外周血T淋巴细胞及脾脏B淋巴细胞的增殖能力.结果显示,提取的FSP均具有多糖的特征峰,为多糖类化合物.外周血T淋巴细胞增殖试验表明,除FSP50外,所有多糖在一定浓度下均能单独和协同PHA刺激T淋巴细胞增殖,其中FSPt的增殖作用较强.脾脏B淋巴细胞增殖试验表明,除FSP50外,所有多糖在一定浓度下均能单独和协同LPS刺激B淋巴细胞增殖,其中FSP70和FSPt增殖作用较强.综上所述,各级FSP中,FSPt对脾脏B淋巴细胞和外周血T淋巴细胞的增殖作用最强,可以作为进一步研究的材料.