2012-2014年我国鸡传染性支气管炎分子流行病学调查

摘要

为了解近年来我国鸡传染性支气管炎病毒的主要类型，2012—2014年，从华东、华北和华中等地区出现传染性支气管炎（IB）的养鸡场中，采集病料进行病毒分离。从中分离鉴定出IBV流行株69株。对所有分离株进行S1基因扩增、测序和遗传进化分析。结果显示毒株共包含6个基因型，根据各基因型中包含的毒株数量多少依次为：QX型（54株，占78.26%）、Mass型（6株，占8.7%）、4/91型（3株，占4.35%）、TW-I型（2株，占2.90%）、tl/CH/LDT3/03型（2株，占2.90%）和CK/CH/LSC/99I型（2株，占2.90%）。将GenBank中收录的所有2012—2014年间国内分离株S1基因序列下载并构建遗传进化树，结果表明QX型IBV已成为当前国内流行的绝对优势基因型。%In order to investigate the epidemiology of the avian infectious bronchitis(IB)strains in China,the infected samples of sick/dead chickens in suspected IB farms were collected from various provinces in the east,north and central area of China from 2012 to 2014. By virus isolation test,69 IB isolates were identified. The S1 gene of all isolates were sequenced and conducted by the phylogenetic analysis.The results showed that there were six genotypes:QX genotype (54 isolates,78.26%),Mass genotype(6 isolates,8.7%),4/91 genotype(3 isolates,4.35%),TW-I genotype(2 isolates, 2.90%),t1/CH/LDT3/03 genotype(2 isolates,2.90%)and CK/CH/LSC/99I genotype(2 isolates,2.90%).All S1 gene se-quences of IB isolates in China from GenBank from 2012 to 2014 were downloaded,and the phylogenetic tree was built up. The results of this study proved that IBV QX genotype was predominant in China.