菊花BRC1b基因的分离与亚细胞定位

摘要

A BRANCHED1-like gene named DgBRC1b was cloned by RT-PCR and rapid amplification of cDNA ends（RACE） using Dendranthema grandiflora ＇Jinba＇.A predicated 1 008 bp open reading frame was deduced in the cloned sequence,which encoded 335 amino acids.By alignment analysis,the deduced amino acid sequence contained two conserved regions which was identified as diagnostic of true TCP.The gene was proposed to belong to TB1 subfamily by phylogenetic analysis.Subcellular localization revealed that DgBRC1b was localized preferentially distributed to nucleus.%以切花菊‘神马’（Dendranthema grandiflora‘Jinba’）为材料,利用同源基因克隆法结合RACE技术克隆了侧枝相关重要基因BRANCHED1类似基因,命名为DgBRC1b。该基因开放阅读框有1 008bp,可编码335个氨基酸。蛋白对比发现,DgBRC1b所推测的氨基酸序列包含TCP家族转录因子的2个基本结构域。通过系统遗传进化树分析进一步表明DgBRC1b属于TB1亚家族。洋葱表皮亚细胞定位结果表明该基因定位于细胞核,进一步证明该基因可能为转录因子。