两步实验设计优化栅藻产油条件的工艺研究

摘要

提出了一种简单、快速优化栅藻(Scenedesmus obliquus)产油条件的两步实验设计方法.首先,利用部分析因设计方法从6个因素(光照强度,温度,pH值,接种量,葡萄糖浓度,氮源浓度)中筛选出显著因素.再利用正交试验进一步确定出最优条件.结果表明:氮源浓度和温度是影响栅藻产油的显著因素.适合培养栅藻产油的最优条件为:氮源6.0 mmol/L,温度25℃和光照80μmol/(m2·s),最高油脂产率达到75.46 mg/(L·d).%A fast and easy two-step statistical optimization of cultivation condition for producing lipid by Scenedesmus obliquus was described.At first,a fractional factorial design (FFD) was used to screen significant factors from six factors ( illumination,temperature,pH value,inoculation,glucose concentration and nitrogen source concentration).Then an orthogonal experiment was executed to further determine optimal conditions.According to the results,nitrogen source concentration and temperature were selected as significant factors.The optimal conditions occurred at nitrogen source concentration 6.0 mmol/L,temperature 25 ℃ and illumination 80 μmol/( m2 · s),with the highest lipid productivity of 75.46mg(L·d).