对药用植物火炭母(Polygonum chinense Linn.)提取物的抑菌活性进行了研究.结果表明:(1)不同部位提取物中,叶和茎均含有抑菌活性物质,但叶提取物的抑菌效果更强、更明显；(2)不同溶剂提取物中,65％乙醇提取物具有最佳抑菌效果；(3)不同提取方法提取物中,超声提取物抑菌效果较索氏提取物抑菌效果强,但差异不是很大；(4)不同溶剂萃取物的抑菌实验表明,火炭母提取物中的抑菌活性物质易溶于乙醚、石油醚等非极性溶剂；(5)火炭母提取物对金黄色葡萄球菌、痢疾杆菌、枯草杆菌、藤黄球菌、白色念珠菌的最小抑菌浓度(MIC)分别为0.6 g·mL-1、0.6g·mL-1、0.8g·mL-1、1.0g· mL-1、0.6 g·mL-1,最小杀菌浓度(MBC)均为1.0g·mL-1.为开发新型、安全、高效的临床药物及扩大药源提供了科学依据.%The antimicrobial activity of the extract from Polygonum chinense Linn, was studied in this paper. The results were as follows:(1) The antimicrobial substances existed in the stems and leaves , and antimi-crobial activity of leaf extract was better than that of stem extract. (2)The antimicrobial efficiency of the extract using 65% ethanol solution was the best. (3)The antimicrobial efficiency of ultrasonic extract was better than that of Soxhlet extract,but there had a little difference. (4)The bacteriostatic test by different polar solvents showed that the antimicrobial substances easily dissolved in non-polar solvent such as ethyl ether and petroleum ether. (5)The minimal inhibitory concentrations(MIC) of the extract for Staphylococcus aureus ,Shigella dys-enteriae,Bacillus subtilis , Micrococcus luteus, Monilia albicans were 0. 6 g·mL-1, 0. 6 g·mL-1, 0, 8 g·mL-1,1.0g·mL-1,0.6g·mL-1, respectively, and the minimal bactericidal concentrations(MBC) all were 1. 0 g · mL-1. The results provided a basis to develop novel, safety, high-efficiency drug and extend the drug resource.
展开▼
