PEI-PEG接枝壳聚糖对于细胞SMMC7721转染效率的研究

摘要

In this study,nanoparticles grafting polycationic polyethylenimine( PEI) and methoxy poly( ethylene glycol) ( mPEG) onto chitosan,mPEG-O-CS-PEI,as potential gene carriers for targeting SMMC7721 cells was investigated. DNA-mPEG-O-CS-PEI nanop-articles transfected 7. 1%of the SMMC7721 cells. These indicated that mPEG-O-CS-PEI was a promising effective gene delivery and package molecule.%本文通过聚乙二醇化的聚乙烯亚胺与壳聚糖反应，得到了水溶性良好的聚乙二醇化聚乙烯亚胺接枝的壳聚糖( mPEG-O-CS-PEI)。研究表明，纳米mPEG-O-CS-PEI对细胞SMMC7721的转染效率为7．1%。