本文建立了以铁氰化钾-Fe（III）体系测定盐酸异丙肾上腺素的方法。研究表明，在pH 3.0，盐酸异丙肾上腺素可使Fe（III）还原为Fe（II），生成的Fe（II）可以与K3[Fe（CN）6]反应生成可溶性普鲁士蓝（KFeI I [FeI （CN）6）。盐酸异丙肾上腺素的浓度在0.03～5.00μg·mL－1范围内与吸光度呈现良好线性关系，线性回归方程A＝0.017＋0.57779C（μg·mL－1），相关系数R＝0.9997，检出限为0.025μg·mL－1，相对标准偏差R. S. D.＝1.05％（n＝11），间接测定盐酸异丙肾上腺素的摩尔吸光系数ε＝1.4×105 L·mol－1·cm－1。本方法成功用于药物制剂和生物样品中盐酸异丙肾上腺素含量的测定，结果满意。%A novel method has been established to determine isoprenaline hydrochloride by potassium ferricyanide-Fe(III). In the presence of potassium ferricyanide,it has been demonstrated that Fe(III)is reduced to Fe(II)by isoprenaline hydrochloride at pH 3. 0,and the in situ formed Fe(II)reacts with potassium ferricyanide to form soluble prussian blue(KFeI I [FeI (CN)6 ]). Beer’s law is obeyed in the range of isoprenaline hydrochloride concentrations of 0. 03-5. 00μg · mL-1 at the maximal absorption wave-length of 735 nm. The linear regression equation is A=0. 017+0. 57779C(μg·mL-1 )with a correlation coefficient of 0. 9997. The detection limit(3σ/k)is 0. 025 μg·mL-1 ,and R. S. D. is 1. 05%(n=11). Moreover,the apparent molar absorption coefficient of indirect determination of isoprenaline hydrochloride is 1. 4×105L·mol-1·cm-1. The parameters with regard to determination are optimized,and the reaction mechanism is discussed. This method has been successfully applied to determine isoprenaline hydrochlo-ride in pharmaceutical and biological samples. Analytical results obtained with this method are satisfactory.
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