利用Nafion与聚丙烯酸钠的混合膜包覆三联吡啶钌（Ru（bpy）32＋）掺杂SiO2纳米粒制备了用于生物标记的电化学发光纳米粒，并构建了信号放大的免疫检测探针。结合免疫磁珠构建的捕获探针，建立了乙肝表面抗原的双抗体夹心电化学发光免疫检测方法。该方法的线性范围为5.0～1000pg·mL－1，相关系数R2为0.9959，最低检测限为1.73 pg·mL－1（S／N＝3），表现出了高达200倍浓度差的宽线性检测范围和高灵敏度。%The electrochemiluminescence(ECL)nanoparticles as bio-label were prepared by using Nafion-sodium polyacrylate mix-ing-membrane covered Ru(bpy)3 2+–doped SiO2 nanoparticles. Accordingly,the immuno-detection probes with ECL signal amplifi-cation have been successfully constructed. Based on the detection probes,this paper has established the double antibody sandwich ECL immuno-detection method for Hepatitis B surface antigen in the combination with the prepared magnetic bead-based immuno-capture probe. The linear range was 5. 0 ~1000pg · mL-1 with correlation coefficient0. 9959 (R2 ). The lowest detectable limit was1. 73 pg·mL-1(signal-to-noise 3 ). The method has a wide linear range with up to 200 times of the detected concentration difference and a high sensitivity for Hepatitis B surface antigen detection.
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