基于环境污染物对DNA损伤筛查的电化学发光传感器的构建

摘要

In this article,a DNA damage sensor was further developed by using[Ru(bpy)2dppz]2+ as electrochemiluminescence ( ECL) indicator. The thiol-labeled dsDNA was assembled on a Au electrode via Au-S bond and then the electrode was immersed in [Ru(bpy)2dppz]2+ solutions. A[Ru(bpy)2dppz]2+/DNA/Au ECL sensor was obtained based on[Ru(bpy)2dppz]2+ as dsDNA molecular“light switch”. Moreover,the experimental conditions,such as[Ru(bpy)2dppz]2+ soaking time,soaking concentrations were optimized. The ECL intensity measured in substrate solution containing[ Ru( bpy) 2 dppz] 2+ increased five times than the sub-strate solution absence of[ Ru( bpy) 2 dppz] 2+. What’s more,the ECL intensity was significantly reduced when the sensor was incuba-ted in pentabromodiphenyl ether,styrene oxide,2-aminobenzimidazole,quinclorac solutions,indicating that the prepared sensor can be used for environmental pollutants screening measurement of DNA damage.%在本文中,进一步发展了以二联吡啶二吡啶并[3,2-a：2′,3′-c]吩嗪钌([Ru(bpy)2dppz]2+)为电化学发光指示剂的DNA损伤传感器。将通过S-Au键组装在金电极表面的双链DNA浸泡在[Ru(bpy)2dppz]2+溶液中,利用[Ru(bpy)2dppz]2+是双链DNA分子“光开关”的特性,得到[Ru(bpy)2dppz]2+/DNA/Au电化学发光传感器,并优化了[Ru(bpy)2dppz]2+的浸泡时间,浸泡浓度等实验条件。发现在含有[Ru(bpy)2dppz]2+的底液中测定其电化学发光强度值比不含[ Ru( bpy)2 dppz]2+的底液中增强了6倍左右。此外将此传感器经全氟辛酸磺酸、五溴联苯醚、氧化苯乙烯等物质温浴后,其电化学发光强度显著降低,说明该传感器可用于环境污染物对DNA损伤的筛查测定。