基于氨基化碳量子点荧光猝灭法检测苏丹Ⅳ的研究

摘要

通过一步水热法以葡萄糖为原料合成稳定性高的碳量子点,并用氨水使其表面氨基化.苏丹Ⅳ能使氨基化的碳量子点荧光明显猝灭,由此建立一种简便、高效检测苏丹Ⅳ的新方法.考察了缓冲体系pH、反应时间、反应温度等对苏丹Ⅳ测定的影响.结果表明,在pH=8.4的硼酸-硼砂缓冲溶液中,室温反应30min时,体系的F0/F与苏丹Ⅳ浓度呈良好的线性关系,其线性范围为2.0×10-6 mol·L-1～9.0×10-5 mol·L-1,相关系数R2=0.9913,检出限为6.4×10-7mol·L-1.方法用于食品中苏丹Ⅳ含量的检测,加标回收率为90.0％～102.5％.%With crystal giucose as raw material,the carbon quantum dots (CQDs)and amino-functional carbon quantum dots (NCQDs) were synthesized through one-step hydrothermal method.Based on the significant quenching effect of Sudan Ⅳ on the fluorescence of N-CQDs,a method with high sensitivity and good selectivity for the determination of Sudan Ⅳ was established Different influencing factors were studied including pH buffer system,reaction time,reation temperature and so on.The results show that,in borate-borax buffer solution of 8.4,the reaction of room temperature 30 min,the ratio of fluorescence intensities(F0/F) and Sudan Ⅳ concentration had good linear relationship,the linear range is 2.0× 10-6 mol · L-1 ～9.0× 10-5 mol · L-1,the correlation coefficient R2 =0.9913,the detection limit is 6.4 × 10-7 mol · L-1.The method is simple and applicable to the determination of Sudan Ⅳ in food samples with satisfactory results,the recoveries were 90.0％ to 102.5％.