角蛋白组氨酸金属结合体的制备及其对SOD酶的模拟

摘要

The water-soluble biopolymer,feather keratin combining with histidine metal ions complexes(FK-HisMx ),were prepared by the soluble feather keratin(FK)conjugating with histidine and metal ion(CuI ,ZnI ,NiI ,MnI ). The structures of FK-HisMx were characterized by UV-Vis,IR,TGA,CD and SEM. The superoxide anion radical(O2 ·-)scavenging activity of FK-HisMx was investi-gated by NBT photochemical reduction and the effect mechanism of scavenging O2 ·-was also explored. The results show that the conjugates with different metal ions had antioxidant activities. After combining with the biopolymer FK,the Cu conjugate (FK-HisMx )shows the most excellent scavenging ability of superoxide anion radical(O2 ·-),EC50 was 0. 0115μmol/L and its simulated degree reached 356. 5%for nature Cu,ZnSOD.%本文将羽毛角蛋白(FK)与组氨酸(His)复合,再与金属离子M(CuI、ZnI、NiI、MnI)络合成功制得了角蛋白组氨酸金属结合体FK-HisMx(M=Cu、Zn、Ni、Mn).采用紫外光谱(UV-Vis)、红外光谱(IR)、圆二色谱(CD)、热重分析(TGA)、扫描电镜(SEM)等手段对FK-HisMx(M=Cu、Zn、Ni、Mn)的结构进行了表征.用核黄素/DL-甲硫氨酸-NBT还原法考察了FK-HisMx对超氧阴离子自由基(O2·-)的清除作用,研究了其清除机理.结果发现:FK-HisMx均具有清除O2·-的能力.其中,FK-HisCux具有较高的O2·-清除活性,FK-HisCu60的O2·-清除活性最好,其抗O2·-的EC50值为0.0115μmol·L-1,对Cu,ZnSOD的模拟度高达356.5%.