ESI-MS法研究N-苯甲酰基-脱氢枞胺衍生物与血管紧张肽Ⅲ的相互作用

摘要

The interactions between the novel N-benzoyl-dehydroabietylamine derivatives (NBDD)including N-benzoyl-dehydroabietylamine ( NBD ), N-o-chlorobenzoyl-dehydroabietylamine ( NCIBD ), N-benzoyl-dehydroabietylamine-7-one (NBDO) and N-o-chlorobenzoyl-dehydroabietylamine-7-one ( NCIBDO ) with angiotensin Ⅲ (AngⅢ) were investigated by electrospray ionization mass spectrometry(ESI-MS). The results show that when the four derivatives respectively mixed with Ang Ⅲ in molar ratios of NBDD: AngⅢ 1: 1, 3:1 and 5: 1, the non-covalent complexes with binding stoichiometries of 1:1 NBDD-AngⅢ and 2:1 (NBDD)2-AngⅢ formed and the complexes mainly formed + 2 charged ions [ NBDD-Ang Ⅲ+ 2H ] 2 + and [ (NBDD) 2-Ang Ⅲ +2H ] 2 +. The stability order of the four kinds of complex ions of binding stoichiometries of 1: 1 [ NBDDAng Ⅲ + 2H ] 2 + was NBDO-Ang Ⅲ＞ NCIBDO-Ang Ⅲ ＞ NC1BD-Ang Ⅲ ＞ NBD-Ang Ⅲ. And for the complexes of binding stoiehiometries of 2: 1 (NBDD) 2-Ang Ⅲ, the combination mode of two NBDD molecules with Ang Ⅲ was NBDD-Ang Ⅲ -NBDD.%采用电喷雾质谱法研究了4种新合成的N-苯甲酰基-脱氢枞胺衍生物(NBDD)--N-苯甲酰基-脱氢枞胺(NBD)、N-邻氯苯甲酰基-脱氢枞胺(NClBD)、N-苯甲酰基-脱氢枞胺-7-酮(NBDO)和N-邻氯苯甲酰基-脱氢枞胺-7-酮(NClBDO)与血管紧张肽Ⅲ(AngⅢ)的相互作用.结果表明,这4种化合物分别与AngⅢ以摩尔比1∶1,3∶1和5∶1混合时,均可形成化学结合计量比为1∶1和2∶1的非共价复合物,这些复合物主要电离为+2价离子[NBDD-AngⅢ+2H]2+和[(NBDD)2-AngⅢ+2H]2+.化学结合计量比为1∶1的NBDD-AngⅢ复合物+2价离子的稳定性顺序为:NBDO-AngⅢ>NClBDO-AngⅢ>NClBD-AngⅢ>NBD-AngⅢ; 在化学结合计量比为2∶1的(NBDD)2-AngⅢ复合物中,2个NBDD分子与AngⅢ的结合形式为NBDD-AngⅢ-NBDD.