采用退火处理方法调控电纺聚左旋乳酸(PLLA)纤维的刚度,研究了纤维刚度变化对诱导多能干细胞来源的间充质干细胞(iPS-MSCs)的形貌、增殖和迁移行为的影响.结果表明,退火处理对纤维的直径影响不大[退火前(1.26±0.25)μm,退火后(1.24±0.26)μm],但提高了纤维结晶度和力学性能,导致纤维刚度增加1.73倍.将iPS-MSCs在退火处理的纤维支架上培养24 h后的细胞骨架铺展面积及7 d后的细胞增殖分别是未处理前的1.78和1.18倍.纤维刚度的提高影响iPS-MSCs的迁移并促进迁移相关基因Integrinβ1, RhoA和Rock1的表达上调.通过退火处理提高电纺PLLA纤维刚度可作为仿生支架构建中调控细胞生物功能的一个重要手段.%The stiffness of as-electrospun poly-L-lactic acid(PLLA)fibers was altered by annealing treatment, and then influence of fiber stiffness on the morphology, proliferation and migration of induced pluripotent stem cell-derived mesenchymal stem cells(iPS-MSCs)were evaluated. The results showed that annealing treatment had negligible influence on the diameter of PLLA fibers[before:(1.26±0.25)μm and after:(1.24 ± 0.26)μm]. However, fiber crystallinity and mechanical properties were enhanced, which resulted in 1.73 times stiffer than that of fibers before annealing treatment. The cell spreading area and proliferation of iPS-MSCs cultured on the treated PLLA scaffold after 1 and 7 d of incubation were 1.78 and 1.18 times higher,respectively, than that of untreated scaffold. The increase of fiber stiffness can affect the migration of iPS-MSCs and promote up-regulated expression of the migration-related genes Integrinβ1, RhoA and Rock1. Collectively,this study demonstrated that increasing fiber stiffness by annealing may be used as an important modality to regulate cell biological functions of the cells in engineering tissues.
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