脱氧胆酸修饰的银纳米粒子比色法检测H2PO4-

摘要

It is of extensive interest in developing a simple,selective,sensitive and rapid approach for anions sensing,as anions play a significant role in biological systems and environment. A simple and sensitive detection method based on the deoxycholic acid modified silver nanoparticles has been developed for the determination of H2PO4–in aqueous solution. The deoxycholic acid modified silver nanoparticles were synthesized by reduction method with sodium borohydride. The research indicates that those Ag NPs allowH2PO4–to be visually detected with high selectivitybecause of the hydrogen bonding interaction between H2PO4–and the ligand. Moreover,the assay method showed a linear response range of 6×10–7—6×10–6mol/L for H2PO4–with colorimetric detection limit being 7×10–7mol/L,which allows the method to be applied for the colorimetric detection of H2PO4–.%阴离子在生命科学和环境科学等领域发挥重要的作用，建立快速、高效的阴离子检测方法越来越被关注。本文基于脱氧胆酸修饰的银纳米探针建立一种简便快速检测 H2PO4–的比色方法。采用硼氢化钠还原法合成脱氧胆酸修饰的银纳米粒，将该银纳米粒用于对阴离子的比色检测。结果表明，通过银纳米粒表面的配体脱氧胆酸与 H2PO4–之间较强的氢键作用，H2PO4–诱导银纳米粒颜色的改变，HPO42–等其他阴离子并未发生明显变化，从而实现对 H2PO4–高选择性的比色识别。该脱氧胆酸修饰的银纳米粒探针检测 H2PO4–的线性范围为6×10–7～6×10–6mol/L，比色检测限为7×10–7mol/L，可以在水溶液中实现对H2PO4–的检测。