响应面法优化决明子水溶性多糖提取条件研究

摘要

Response surface methodology was used tooptimize the ultrasonic extractionof polysaccharides fromcassia seeds. RSM was employed to evaluate the interactive effects and significanceof threecrucial variables including extraction time,ultrasonic power and extraction temperature,mathematical model for the yieldof polysaccharides fromcassia seeds as function was established. Theoptimumconditions were extraction timeof 36.5 min,ultrasonic powerof 233 W and extraction temperatureof 51℃. Under theoptimizedconditions,the yield was up to 10.74%,which was inclose agreement with value predicted by the model.%利用响应面法优化超声波提取决明子水溶性多糖的最佳工艺。考虑了三个单因素提取时间、超声功率和提取温度，以决明子水溶性多糖得率为响应面值，做三因素三水平响应面回归分析。通过分析因素的显著性和相互作用，优化得到了提取最佳工艺条件为提取时间36.5 min、超声功率233 W、提取温度51℃，其多糖得率为10.74%，其实际结果与预测值比较接近。