利用枯草芽孢杆菌、凝结芽孢杆菌、乳酸杆菌对豆粕进行生料混菌发酵,制备生物活性饲料.通过单因素和响应面分析试验,以三氯乙酸可溶性氮(TCA-NSI)为优化指标对其发酵工艺条件进行研究,得出最佳发酵条件为:枯草芽孢杆菌与乳酸菌之比为2.2∶1、发酵温度30℃、外加水量46.5%、总接种量15 ml(在豆粕72 g、麸皮8 g的发酵基质中)、拌料水pH值自然.在此条件下发酵84 h,测得三氯乙酸可溶性氮含量为12.78%.%Raw soybean meal was fermented by the strains mixture of Bacillus subtilis, Bacillus coagulans and Lactobacillus to produce bioactive feed. The fermentation operation conditions were studied via single-factor experiments and response surface analysis and with the content of trichloroacetic acid soluble nitrogen (TCA-NSI) as the optimal index. Finally the optimal fermentation operation parameters were obtained as follows: inoculum ratio of Bacillus bacteria to Lactobacillus 2.2∶1, fermentation temperature 30℃, amount of water added 46.5 ％, total inoculum dose 15 mi(fermentation matrix of soybean meal 72 g and bran 8 g) and the pH of mixed water was natural. Under those conditions, the TCA-NSI content reached 12.78％ after a 84 h fermentation process.
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