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《中国免疫学杂志(英文版)
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>Ex Vivo Stimulation of Tumor-Draining Lymph Node Cells from Lung Cancer Patients:A Potential Resource for Adoptive Immunotherapy
Ex Vivo Stimulation of Tumor-Draining Lymph Node Cells from Lung Cancer Patients:A Potential Resource for Adoptive Immunotherapy
To find a feasible method for the stimulation of tumor-draining lymph node (TDLN) cells in preparation for use in the clinic,the CTL activity of TDLN cells induced by different stimuli (IL-2 alone,IL-2 + autologous tumor antigen (atAg),IL-2 + GM-CSF + IL-4 + atAg) was measured by maximal LDH enzyme release. The mechanisms were explored by the observation of morphology and the detection of CD83+ TDLN cells. The expansion of TDLN cells by IL-2 + GM-CSF + IL-4 + atAg was significantly higher than that by IL-2 alone or IL-2 + atAg (p < 0.01). Antitumor CTL activity of TDLN cells induced by IL-2 + GM-CSF + IL-4 + atAg was significantly higher than those of other groups. The number of CD83+ cells within the TDLN population treated with IL-2 + GM-CSF + IL-4 + atAg was significantly elevated. The method of stimulating TDLN cells by IL-2 + GM-CSF + IL-4 + atAg was better than the stimulation with IL-2 or IL-2 + atAg. TDLN cells induced by IL-2 + GM-CSF + IL-4 + atAg produced more dendritic cells (DCs). In our study,we established a system that T cells and DCs were stimulated together ex vivo,which was easy to conduct and produce promising results. It provided a new method for improving TDLN cell antitumor activity which might be used in the clinical cancer therapy.
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机译:Adoptive Immunotherapy of Cancer with Activated Lymph Node Cells Primed In Vivo with Autologous Tumor Cells Transduced with the GM-CSF Gene University of Michigan, Ann Arbor, Michigan
机译:Tumor-specific granulocyte/macrophage colony-stimulating factor and interferon γ secretion is associated with in vivo therapeutic efficacy of activated tumor-draining lymph node cells
机译:Asynchrony of primary tumor and mediastinal lymph nodes response after neoadjuvant immunotherapy plus chemotherapy in a patient with stage IIIA non-small-cell lung cancer: a case report
机译:Stereotactic Ablative Radiotherapy versus Lobectomy for Operable Stage I Non-small-cell Lung Cancer: A Pooled Analysis of Two Randomized Trials——胸外科医生的解读
机译:Suspicious Cervical Lymph Nodes Detected after Thyroidectomy for Papillary Thyroid Cancer Usually Remain Stable Over Years in Properly Selected Patients