聚乙交酯丙交酯共聚物体外实时降解和加速降解的相关性研究

摘要

Objective To observe the relationship of the real-time degradation test and accelerated degradation test with PLGA sample.Method PLGA samples were immersed in a Sorensen buffer solution at 37℃ and 50℃ separately for a period of time. At the specified time point, the sample, debris and solution were separated with a filter and tests such as tensile strength, mass loss and intrinsic viscosity were carried out. Meanwhile, the degradation products in the solution were determined. Results mass loss percentage and intrinsic viscosity reduction of accelerated degradation was significantly greater than that of real-time degradation. The mass loss percentage both in real-time degradation and accelerated degradation showed a linear correlation while the intrinsic viscosity percentage both in real-time degradation and accelerated degradation showed a logarithmic relationship. ConclusionThere were some relationships between accelerated degradation test and real-time degradation test. Accelerated degradation rate is about 3.5~4 times of the real time degradation rate in this study.%目的：通过对聚乙交酯丙交酯共聚物（PLGA）体外实时降解和加速降解的试验研究，考察两种降解过程的相关性。方法实时降解试验是将PLGA样品浸泡于Sorensen缓冲液中，于37℃条件下放置不同的时间，加速降解试验是将PLGA样品浸泡在Sorensen缓冲液中，于50℃条件下放置不同的时间，到指定时间点时，分离样品和降解液，测试样品主体的抗张强度、质量损失和特性黏度，并测试降解液中降解产物的含量。结果加速降解的质量损失百分比和特性黏度的减小是明显大于实时降解的，实时降解和加速降解的质量损失百分比均呈一定的线性相关关系，实时降解和加速降解的特性黏度百分比呈现对数关系。结论加速降解和实时降解具有一定的相关性，本试验发现，加速降解速率约是实时降解速率的3.5～4倍。