几种cDNA差减文库构建方法的比较

摘要

cDNA subtractive library is one of the best method which be used to analyze gene differential expression and decrease the scope in gene selection . The methods in cDNA subtractive library construcdon have been reported a lot, and also developed two kinds of directions , btt no one is perfect still now. In this paper , the principle ,advantages and disadvantages of those methods are discussed.%cDNA差减文库的构建是研究基因差异表达、缩小目的基因筛选范围的理想方法。近年来报道了多种构建方法，并形成了两个发展趋势，但目前还无一种方法被普遍认可。现就几种常见方法的原理、思路及优缺点进行了比较，以供借鉴。