羟基红花黄色素A对脂多糖作用后血管内皮细胞诱导型一氧化氮合酶表达的影响

摘要

It was to investigate the effect of hydroxysafflor yellow A( HSYA) on the expression of lipopolysaccharide (LPS)-elicited inducible nitric oxide synthase (iNOS) in vascular endothelia1 cell line HUVECs.Human umbilica1 vein endothelial cells HUVECs were induced by 1 mg/L LPS and HSYA with the concentration of 0.01,0.1,1 mmol/L respectively.Cell survival rate was measured by MTT assay. Nitric oxide (NO) level was detected by nitrate reductase method. The iNOS expression was detected by RT-PCR and Western blotting. Results showed that there was no significant change of LPS-elicited iNOS expression with 0.01,0.1mmol/L HSYA, while 1 mmol/L HSYA could obviously inhibite the expression of iNOS induced by LPS.It can conclud that HSYA can inhibit LPS-elicited iNOS expression and NO production in HUVECs cells, which may be used in the treatment of vascular inflammation.%旨在探讨羟基红花黄色素A(hydroxysafflor yellow A,HSYA)对脂多糖(lipopolysaccharide,LPS)作用后人脐静脉内皮细胞株HUVECs细胞株诱导型一氧化氮合酶(inducible nitric oxide synthase,iNOS)表达的影响.培养HUVECs细胞株,用 1 mg/L LPS及不同浓度的HYSA处理细胞24 h,MTT法检测细胞增殖情况,硝基还原酶法检测培养液中一氧化氮(NO)含量,RT-PCR及Western blotting检测iNOS表达.结果表明0.01、0.1 mmol/L HYSA对LPS引起的iNOS升高无明显作用,但1 mmol/L HYSA能明显抑制LPS作用后高度表达的iNOS量.因此,HYSA能下调LPS所致iNOS的异常表达,这可能有助于临床治疗血管炎症疾病.