大伏革菌产纤维素酶条件优化及高效菌株筛选

摘要

The activity of extracellular cellulase could be used for screening the effective biocontrol strain. We could acquire the most effective Phlebiopsis gigantea isolate associated with biocontrol conifer root and butt rots by determining cellulase activity of them. Based on single factor and orthogonal experiment, the optimum medium compositions for the highest cellulase activity were(g/L):glucose 30.00, peptone 5.00, KH2PO4 3.00, MgSO4·7H2O 1.50, the optimal liquid medium volume was 120 mL/250 mL, the optimal inoculum volume was 5%(V/V), initial pH4.0. By determining cellulase activity of P. gigantea isolates, we concluded that P. gigantea 08077 had the highest cellulase activity and 13025 was the lowest. P. gigantea 08077 is better to control the Chinese Heterobasidion parviporum than Rotstop-F that had been registered in Finland.%真菌胞外纤维素酶活可作为高效生防菌株筛选的指标之一，通过测定野生大伏革菌（Phlebiopsis gigantea）菌株在最优条件下所产生纤维素酶活力高低来达到筛选防治针叶树根腐病高效菌株的目的。以大伏革菌菌株液体培养过程中纤维素酶活力为指标，通过单因素和正交试验结合的方法，筛选出大伏革菌最适产纤维素酶条件为（g/L）：葡萄糖30.00，蛋白胨5.00，磷酸二氢钾3.00，硫酸镁1.50，装液量120 mL/250 mL，接种量5%（V/V），初始pH为4.0。测定野生大伏革菌菌株纤维素酶活力的大小显示：08077号菌株纤维素酶活力最大，13025号菌株最小。研究得出08077号菌株比芬兰商业化生物制剂Rotstop-F具有更好的防治中国小孔异担子菌的潜能。