以武夷菌素生物合成正调控基因wysR过表达菌株为初选材料，通过琼脂块法对2060个过表达菌株单菌落进行初筛，得到35个优选菌株，再采用摇瓶发酵复筛的方法筛选出9个高产菌株，并通过菌株遗传稳定性分析，最终确定W-273菌株为最优菌株。研究武夷菌素wysR基因过表达菌株W-273的生理生化特征，发现与原始菌株CK-15相比，W-273菌株生长迅速，产孢量增加且产孢时间提前，W-273菌株生长3-4 d即可完成产孢，较菌株CK-15产孢时间提前了3-4 d；电子显微镜观察W-273菌株和CK-15菌株孢子形态不同，W-273菌株孢子呈椭圆形或杆状，CK-15菌株孢子呈圆形；通过摇瓶发酵表明W-273菌株较CK-15菌株代谢旺盛，抗生素产量提高79%-289%，且最佳发酵时间缩短4-8 h。%In this research the over-expression strains with positive-regulating genewysR synthesized from Wuyiencin were utilized as primary materials, 35 relatively optimal strains were selected from 2060 individual colonies of over-expression strains by agar blocks, then 9 high-yield strains were screened out adopting the method of shake flask fermentation. Ultimately, the strain W-273 was determined as the optimal strain by genetic stability analysis. Comparing the physiological and morphological characteristics between the strain of W-273 and original CK-15, W-273 strain grew faster, the production of spore was higher, and the time of spore production was in advance. The time to complete spore production for W-273 strain was 3-4 d, and this was in advance for 3-4 d than the original strain CK-15. Through electron microscope observation, the spores of W-273 were oval or rod, and the CK-15 strains were circular. Shake flask fermentation showed that strain W-273 had a higher metabolism compared to strain CK-15, antibiotic production increased 79%-289%, and the best fermentation time shortened 4-8 h.
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