细胞质雄性不育系转录组学研究进展

摘要

细胞质雄性不育是植物杂种优势利用的基础，也是研究线粒体基因与核基因之间相互作用的良好材料。采用 DNA芯片和 RNA 测序技术进行转录组分析能够在同一个实验中分析和比较成千上万个基因，这为在基因组水平上更好地了解植物生长和发育机制以及遗传变异提供了可能。在细胞质雄性不育机理的研究上，通过转录组分析将获得详细的相关分子分析的信息，了解线粒体与核基因之间的相互调控作用方式，并有助于雄性败育机理的解析。%Cytoplasmic male sterility(CMS)is the basis of utilizing the heterosis of plants,and also favorable material for studying the interaction between mitochondrial genes and nuclear genes. Transcriptomics analysis using DNA microarray and RNA-Seq technology enabled the analysis and comparison of thousands of genes in one experiment. This allows it feasible to better understand fundamental aspects of growth and development,as well as genetic variation in plants on a genomic scale. Based on the mechanism of CMS lines,transcriptomics study may acquire the detailed relevant molecular analysis,understand the interaction mode between mitochondrial and nuclear genes,and be conducive to dissect the male sterility.