韭菜胚根高效植株再生体系的建立

摘要

In order to establish the high frequencey regeneration system of Allium tuberosum, using the radicle as the explant, the paper examined callus and bud induction from its various parts. The results showed that the radicle tip was the best explant for inducing callus with the induction rate of 81.4%, and the bud induction rate reached 73.3%. Comparing the induction of callus and differentiation of bud in several media formulas, the regeneration rate was the highest in medium of MS+1 mg/L BA+0.5 mg/L NAA+0.5 mg/L KT, with induction rate over 80% and the differentiation rate of adventitious bud reaching 78.8%, respectively, while the propagation coefficient was 24.7. The percentage of adventitious buds elongating to be independent plant was 85.5% on elongation medium of MS+1 mg/L GA+0.5 mg/L KT at 30 d. The rooting rate and root system for the independent shoots were the best in the medium of 1/2 MS+0.3 mg/L IAA, and in which a single plant from the differentiation of radicle was generated at approximate 130 d.%为了建立韭菜高频植株再生体系，以韭菜胚根的不同部位为外植体，研究了不同部位愈伤组织和芽的诱导。结果表明，胚根尖为诱导愈伤组织的最佳外植体，愈伤诱导率达到81.4%，出芽率达到73.3%；比较在不同激素配方上的愈伤组织诱导和芽的分化，在 MS+1 mg/L BA+0.5 mg/L NAA+0.5 mg/L KT 培养基中再生率最高，诱愈率达到80%以上，不定芽分化率达78.8%，繁殖系数为24.7；在 MS+1 mg/L GA+0.5 mg/L KT 不定芽伸长培养基中，30 d 不定芽伸长为独立植株，比例为85.5%；独立的韭菜苗在1/2 MS+0.3 mg/L IAA 中生根率及根系状况最佳。通过该方法130 d 左右即可从胚根脱分化再生出植株。