Utilization of microalgae for direct fixation of carbon from coal-based flue gas may reduce CO2 emissions,and also produce bio-based products with multiple values. However,CO2,NOx and SO2 at high concentration in the flue gas always cause excessive acidification of culture medium,leading to severe inhibition of microalgae growth. The present study was conducted to establish a simple and effective method of microalgae domestication for enhancing the microalgae resistance against acid stress and increasing their initial growth rate when inoculated in low-pH medium. The initial growth rate of the acid-domesticated and the UV-treated Scenedesmus reached 0.253 g/(d·L), which was significantly higher than that(0.132 g/(d·L))by only acid domestication at pH4.5 and that(0.092 g/(d·L))of 5-min induction at low-dose of UV radiation in low-pH environment. The more remarkable effects were achieved by combining such acid domestication and UV induction. The domesticated seed algae grew normally in the acidic environment of pH3,but the non-domesticated microalgae growth stopped. The initial growth rate of the domesticated seed algae was 6.6 times higher than that of the non-domesticated microalgae in low-pH environment. The above results revealed that acid domestication and UV induction significantly improved Scenedesmus tolerance against low-pH environment.%应用微藻固定烟道气CO2既可减排又能生产高值生物基产品,但是,烟道气高浓度CO2和氮硫氧化物导致培养液过度酸化、抑制微藻生长.通过建立微藻抗性驯化简易方法,以期提高微藻耐酸性和在低pH条件下的起始生长速率.经过酸驯化和紫外诱导叠加处理后的栅藻(Scenedesmus),在酸性环境下生长初期的平均生长速率达到0.253 g/(d·L),明显高于单因子处理的pH4.5酸驯化(0.132 g/(d·L))和紫外诱导5 min(0.092 g/(d·L))的生长速率.经过驯化处理的种子藻在pH3的酸性环境中可以正常生长,而未驯化微藻生长近乎停滞.经过驯化处理的种子藻接入低pH培养基后起始生长速率比未驯化微藻提高6.6倍.酸驯化和紫外诱导处理显著提高了栅藻对低pH环境的耐受性.
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