四个罗氏沼虾抗病选择系抗病力比较分析

摘要

采用荧光定量PCR方法,对4个罗氏沼虾抗病选择系的4个抗病力基因(热休克蛋白70(HSP70)Se-GPx、CAT、SOD)表达量分析.通过感染溶藻弧菌(Vibrio alginolyticus),对比4个选择系之间HSP70、Se-GPx、CAT及SOD表达量的差异,结合4个选择系成活率,来对比4个抗病选择系抗病力强弱.结果表明,A、B两个抗病选择系的肝胰腺HSP70 mRNA的相对表达量明显高于C、D两个抗病选择系(P<0.05).感染96 h内Se-GPx、CAT、SOD表达量变化显著,在4个选择系不同时间表达量也不相同,但是总体变化趋势是A、B选择系高于C、D选择系.结合4个选择系感染溶藻弧菌一周的成活率分别为65.0%、65.8%、51.7%及53.3%,可得出结论A、B两个抗病选择系抗病力高于C、D两个选择系.%Expressions of 4 disease-resistant genes(heat shock protein70(HSP70),Se-GPx,CAT,and SOD)from 4 selected lines(A,B, C,and D)of Macrobrachium rosenbergii were analyzed with real-time PCR. Infected with Vibrio alginolyticus,the disease-resistant abilities of the 4 selected lines were compared based on the survival rates of 4 selected lines and the expression differences ofHSP70,Se-GPx,CAT,and SOD among the 4 selected lines. The results showed that relative expression of HSP70 mRNA of A and B was obviously higher than that of C and D in the hepatopancreas(P<0.05). The expressions of Se-GPx,CAT,and SOD changed significantly within 96 h,and were different among the 4 selected lines at different time ;however,the general trend was that expressions in A and B were higher than those in C and D. The rate of A,B,C,and D was 65.0%,65.8%,51.7%,and 53.3% after infecting with V.alginolyticus for a week,it is inferred that disease-resistant ability of A and B is higher than that of C and D.