不同品种绵羊发情周期LEPRb mRNA表达分析

摘要

为了探讨LEPR在绵羊季节性发情中的表达特点,以季节性发情的中国美利奴和常年发情的多浪羊为研究对象,利用实时荧光定量PCR技术检测繁殖季节不同发情阶段LEPRb mRNA在下丘脑、卵巢和子宫组织中的表达变化.结果显示,在发情间期、前期和发情期中国美利奴卵巢和子宫组织LEPRb的相对表达水平都低于下丘脑,但在发情后期卵巢和子宫LEPRb的相对表达水平显著高于下丘脑.不同发情时期,两品种绵羊同一组织的LEPRb的表达变化幅度有所差异,但趋势相似.且中国美利奴各组织LEPRb的表达水平都高于常年发情的多浪羊.LEPRb的表达变化与绵羊发情存在相关关系.%The aim of the study is to explore LEPR expression characteristics in seasonal estrus of sheep. Chinese merino(seasonal estrus sheep)and Duolang sheep(perennial estrus sheep)were selected to determine the expression changes of LEPRb mRNA in hypothalamus,ovaries and uterus in different stage of estrus cycle by quantitative real-time PCR. The results showed that during the whole estrus cycle,except the metaestrus,the LEPRb levels in the ovarian and uterus were found to be significantly lower than the levels in hypothalamus of Chinese Merino,but the results in metaestrus showed the reverse. Although the expression level of LEPRb varied in the same tissue of 2 cultivars during estrus stage,the trend was similar. Moreover,the LEPRb expression levels in different tissues of Chinese Merino were significantly higher than those in Duolang sheep. The results indicate that the expression variation of LEPRb is correlated with sheep estrus.