橡胶树半胱氨酸蛋白酶抑制剂HbCYS2的克隆与表达分析

摘要

In plant,the cystatin(CYS)is widely involved in growth and development,and responses to biotic and abiotic stresses. In this study,the HbCYS2 is isolated from rubber tree,and has open reading frame(687 bp)encoding a protein of 288 aa. The HbCYS2 is a secreted protein with signal peptide,and has two CYS domains. The bioinformatics analysis indicates that the HbCYS2 is belonged to CYS II(GeneBank :KX161925). The expression analysis indicated that the HbCYS2 has high level expression in latex,and similar expression pattern responding to tapping and ET treatment. In addition,the expression of HbCYS2 is apparently regulated by Corynespora inoculation in leaf. It is speculated that the HbCYS2 plays an important role in latex regeneration,tapping treatment and disease resistance in rubber tree.%半胱氨酸蛋白酶抑制剂(Cystatin,CYS)广泛参与植物的生长发育调控、生物与非生物胁迫应答过程.从橡胶树中获得1个CYS基因,ORF长度为687 bp,编码1个由228个氨基酸残基组成的蛋白,生物信息学分析发现该蛋白含有2个CYS结构域,以及1个含有30个氨基酸残基的信号肽,为分泌蛋白,推测属于植物CYS II型,命名为HbCYS2(GenBank:KX161925).表达分析发现HbCYS2在胶乳中高表达,并且受割胶和产量调节剂ET调控;在叶片组织中,HbCYS2对多主棒孢侵染表现出明显应答.由此推测,胶乳中HbCYS2在橡胶树胶乳再生、以及割胶伤害应答中发挥一定的调控作用,叶片中HbCYS2参与橡胶树棒孢落叶病的防御或抵抗.