间歇出酶/出菌丝的培养方式对里氏木霉产纤维素酶的影响

摘要

The cellulase was produced by fed-batch cultivation of Trichoderma reesei Rut C30 with microcrystalline cellulose and starch hydrolysate as carbon source under the protection of periodic enzyme recovery. The results showed that after three days of fed-batch cultivation,cellulase was partially recovered 15%,30% or 45% at every 1,2 or 3 days. The total enzyme activites of cellulase and β-glucosidase obtained by the methods significantly increased by 26.5%-32.6% and over 46% more than that of fed-bath.Mycelia could also be removed to avoid infection.When enzymes were recovered 15% every day, the cellulase production increased by 35.4% ( without mycelia removal) and 32.5% ( with 15% mycelial removal) compared with those of normal fed-batch cultivation.A high hydrolytic ability of the produced cellulase with enzyme and mycelia recovery was also detected and the hydrolysis efficiency could reach more than 82%,far more than that of Celluclast(62.03%).%以微晶纤维素和淀粉水解液作为碳源生产纤维素酶，在里氏木霉Rut C30分批补料生产纤维素酶的过程中通过间歇取出部分酶的培养方式保护纤维素酶。实验结果显示：采用间歇出酶培养方式，在分批补料3d后，每1、2或者3天取出部分酶液。当平均每天取出15％的酶液，酶活显著增加，总纤维素酶酶活较单纯分批补料提高26.5％~32.6％，而总β-葡萄糖苷酶酶活提高超过46％；采用间歇出酶出菌丝培养方式，当每天取出15％的酶液时，纤维素酶产量比分批补料增长35.4％，去除和酶液等量的菌丝，酶活和分批补料相比增长32.5％，而且这两种培养方式所生产的纤维素酶的酶解效率高达82％，远超商品酶Celluclast(62.03％)。