Objective To investigate the relationship between gene polymorphism of the plasminogen activator inhibi-tor-1 (PAI-1) promotor region 4G/5G gene, angiotensin I converting enzyme gene insertion/deletion (I/D) and cerebral stroke. Methods The genotype of 4G/5G allele polymorphism in the PAI-1 promoter region and I/D allele polymorphism in ACE were determined by polymerase chain reaction with leukocytes from 139 normal controls and 203 patients with cerebral stroke. Serum ACE activity was measured by colorimetry, plasma level of PA 1-1 activity was determined by spectrophotometric assay. Results The PAI-1 and ACE activity in cerebral ischemia group (0. 769 ± 0.163 AU/Ml, 43.42 ± 14. 36 U/L) were significantly higher than those in control group (0.652 ±0.116 AU/Ml, 31.28 ±8.64 U/L, P <0.01). The frequency of PAI-1 4G/4G genotype and 4G alleles, ACE D/D genotype and D alleles in CI group was significantly higher than those in control group(P <0.01). Furthermore, the effects of PAI-1 4G/4G genotype and ACE D/D genotype on cerebral infarction were synergetic (P <0. 01). Conclusion 4G/4G PAI-1 and D/D genotype in ACE is a potential susceptible factor of acute cerebral infarction, 4G and D allele homozy gous genotype may be the major and synergetic risk factors of acute cerebral infarction.%目的 探讨纤溶酶原激活物抑制物-1(PAI-1)启动子区基因多态性和血管紧张素转换酶(ACE)插入/缺失多态性与脑卒中的关系.方法 PCR检测203例脑卒中患者和139名健康对照者PAI-1基因启动子区4G/5G多态性、ACE基因插入/缺失多态性,同时应用比色法测定血清ACE活性,发色底物法测定PAI-1活性.结果 脑梗死(CI)组PAI-1活性(0.769±0.163 AU/mL)、ACE活性(43.42±14.36 U/L)明显高于对照组(0.652±0.116 AU/mL和31.28±8.64U/L,P＜0.01)；CI组PAI-I基因4G纯合子、ACE D/I基因DD纯合子比例明显高于对照组(P＜0.01);PAI-I基因4G/4G基因型与ACE基因D/D基因型对CI发病可相互协同作用(P＜0.01).结论 PAI-1基因4G/4G基因型和ACE基因D/D基因型均可能是CI发病的危险因素,且具有协同作用.
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