骨髓间充质干细胞移植对缺血心肌的血管新生及增殖-凋亡的影响

摘要

Objective To investigate the effects of bone marrow mesenchymal stem cells (BMSC ) transplantation on angiogenesis and proliferation-apoptosis in ischemic myocardium. Methods BMSC isolated from male mice were transfused into female mice with isoproterenol-induced myocardial ischaemia via tail vein. This study included three groups; BMSC, untreated, and control groups. Five weeks after treatment, expression levels of sex-determining region of Y-chromosome (SRY) and vascular endothelial growth factor (VEGF) in myocardium were detected by fluorescent qRT-PCR. Collagen distribution was observed using sirius red staining. The protein expression of VEGF, proliferating cell nuclear antigen(PCNA) and caspase-3 was detected by immunohistochemistry. Results BMSC group had significantly higher expression of SRY (11. 22 ± 0. 90 vs 1. 05 ± 0. 47, P＜0. 05 ). Compared with the untreated group, BMSC group had decreased levels of collagen content(3. 44 ±0. 84 vs 8. 44 ± 1. 09, P＜ 0. 05) and caspase-3 ( 0. 46 ± 0. 11 vs 3. 12 ± 0. 28 , P＜0. 05) , and increased level of VEGF (14. 19 ± 0. 37 vs 11. 88 ± 0. 28, P＜0. 05) and PCNA (4. 08 ± 0. 18 vs 0. 64 ± 0. 05, P＜0. 05). Conclusions BMSC can home to ischemic myocardium. BMSC transplantation improve microvascular angiogenesis and regulate the proliferation andapoptosis in ischemic myocardium.%目的 探讨骨髓间充质干细胞(BMSC)移植对缺血心肌的血管新生及增殖-凋亡的影响.方法 将雄性小鼠BMSC经尾静脉输入异丙肾上腺素性心肌缺血雌性小鼠(BMSC组).另设正常对照组和未治疗组.5周后处死小鼠,荧光定量PCR检测心肌Y染色体鉴别基因(SRY)、血管内皮生长因子(VEGF)的表达.天狼猩红染色分析心肌胶原含量.免疫组织化学染色观察心肌VEGF、核增殖抗原(PCNA)和细胞凋亡蛋白酶(caspase-3)的分布.结果 BMSC组心肌SRY表达明显升高(11.22±0.90 vs 1.05±0.47,P＜0.05).与未治疗组相比,BMSC组的心肌胶原沉积减少(3.44 ±0.84 vs 8.44 ±1.09,P＜0.05),VEGF(14.19±0.37 vs 11.88 ±0.28,P＜0.05)和PCNA(4.08±0.18 vs0.64 ±0.05,P＜0.05)表达上调,caspase-3降低(0.46 ±0.11 vs 3.12 ±0.28,P＜0.05).结论 BMSC能归巢至缺血心肌并促进微血管新生,改善心肌增殖-凋亡状态.