目的:探索腺相关病毒介导的IFNα体外抑制HBV的效果,寻求治疗HBV感染的新策略。方法3种质粒共转染制备重组病毒AAV-IFNα,用AAV-IFNα感染肝细胞系HepG2.2.15,感染后1、3、6和9 d,qPCR检测HBV DNA;ELISA方法检测HBsAg和HBeAg含量;MTT法检测HepG2.2.15细胞增殖的情况。结果 AAV-IFNα可通过抑制HepG2.2.15细胞增殖的方式显著抑制HBV的复制与表达( P<0.05)。结论 AAV-IFNα是一种有潜力的抗HBV的药物。%Objective_To identify the anti-HBV efficiency of AAV-IFNαby infecting HepG2.2.15 cell line, and to develop a new strategy of curing hepatitis B.Methods_Firstly,recombinant vector was packaged into AAV by three plasmids co-transfection method;then AAV-IFNαinfected HepG2.2.15 cell line, and samples were collected on the 1st, 3rd,6th and 9th day;thirdly, The time-course of HBV DNA,HBsAg and HBeAg were detected by qPCR and ELISA.The proliferation of HepG2.2.15 cells detected by MTT.Results_Recombinant AAV-IFNαcan significantly inhibit HBV replication and expression in vitro because of the proliferation of HepG2.2.15 cells inhibited by AAV6-IFNα( P<0.05) .Conclusions_AAV-IFNαis a potential antiviral product for hepatitis B.
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